Figure 1From: Surface-exposed loops L7 and L8 of Haemophilus (Glaesserella) parasuis OmpP2 contribute to the expression of proinflammatory cytokines in porcine alveolar macrophagesmRNA expression levels of proinflammatory cytokines in PAMs stimulated by OmpP2 or the eight loop peptides. PAMs were stimulated with OmpP2 (5 µg/mL and 10 µg/mL), loops L1–L8 or scrambled peptide (130 nmol/mL and 260 nmol/mL) for 6 and 12 h. The mRNA expression levels of IL-1α (A), IL-1β (B), IL-6 (C), IL-8 (D), IL-17 (E), IL-23 (F), CCL-4 (G) and CCL-5 (H) were measured by real-time PCR. The HEPES buffer (pH 7.4) containing 0.25% (w/v) SLS was used as a mock stimulus, and the untreated cells were used as a negative control. Values are presented as the mean ± SD of three independent experiments. Data were analysed using one-way ANOVA. *p < 0.05, **p < 0.01 compared with the negative control.Back to article page