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Figure 2 | Veterinary Research

Figure 2

From: Nature and consequences of interactions between Salmonella enterica serovar Dublin and host cells in cattle

Figure 2

Identification and characterisation of Salmonella-infected bovine cells. Single cell suspensions were prepared from the ileum and LNs of the three calves challenged orally with SD3246-GFP. A Viability of the cells was assessed by flow cytometry. Live leucocytes in the samples were selected followed by identification of SD3246-GFP-infected cells using the GFP signal. The majority of infected cells were found to be MHCII+. The expression of other cell surface molecules was then studied. B The majority of SD3246-GFP-infected cells also expressed CD40, CD80, CD86, CD11b and CD11c but lacked CD1 expression suggesting that they were macrophages. The data shown here are from mesenteric lymph nodes of an infected calf and are representative of infected ileal mucosa and LNs.

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