Figure 7

Serum IgA antibodies (Abs) in PEDV (± VA) gilts increased to higher levels and at a faster rate than mock (± VA) gilts. A Serum PEDV IgA antibody secreting cells (ASCs) and B Ab responses were determined by PEDV ELISPOT and ELISA assay, respectively. C Serum PEDV virus neutralizing (VN) Ab responses were determined by VN assay. D Piglet serum circulating PEDV IgA titers were measured by ELISA. Gilts and piglet serum was collected at piglet post-challenge day (PCD) 0, 5–9, 12–17 and 21–29. Asterisks indicate significant differences among treatment groups at the same time point (mean ± SEM). Statistical analysis was performed using the two-way ANOVA with repeated measures and Bonferroni’s correction for multiple comparisons. *P < 0.05 **P < 0.01 ***P < 0.001.