Figure 6

PEDV antibody (Ab)⁺ cells in the mammary gland (MG) increased post PEDV challenge. A Evaluation of anti-PEDV Ab⁺ plasma cells in the MG pre-partum [gestation day (GD) 104–114] and post-partum [post-challenge day (PCD) 5–9] in mock, mock + VA, PEDV and PEDV + VA gilts by PEDV viral suspension sandwich immunohistochemistry (IHC) method and hematoxylin staining (300×). Right: enlarged view of MG tissue where red arrows indicate PEDV Ab⁺ cells. B Cells were quantified by averaging PEDV Ab⁺ cells from 3 to 6 microscope fields (300×) from different areas of the MG from each animal within mock, mock + VA, PEDV and PEDV + VA gilts. Statistical analysis was performed using the one-way ANOVA. ***P < 0.001.