Figure 5

PEDV + VA gilts had elevated levels of milk PEDV specific IgA ASCs and Abs, and IgA IgSCS. A Milk MNCs were isolated and added to PEDV specific and B, C total ELISPOT plates to determine the PEDV specific IgA ASCs and IgA IgSCs. D Milk MNCs were isolated and analyzed for frequencies of IgA⁺β7⁺ B cells by flow cytometry E PEDV IgA and F virus neutralizing (VN) Abs were determined by VN Ab assay, respectively. Asterisks indicate significant differences among treatment groups at the same time point (mean ± SEM). Statistical analysis was performed using a Mann–Whitney t-test (B–D) or two-way ANOVA with repeated measures and Bonferroni’s correction for multiple comparisons (A, E, F). *P < 0.05 ***P < 0.001.