Figure 3

Circulating PEDV specific IgA ASCs were significantly increased in PEDV + VA gilts pre-partum. A, B Peripheral blood MNCs (PBMCs) were isolated and added to PEDV ELISPOT plates to determine the PEDV specific IgA ASCs. C PBMCs were isolated and analyzed for frequencies of α4⁺β7⁺ B cells and D IgA⁺β7⁺ MNCs by flow cytometry E PEDV IgA Abs were determined by ELISA. Asterisks indicate significant differences among treatment groups at the same time point (mean ± SEM). Frequencies of α4⁺β7⁺ B cells and IgA⁺β7⁺ MNCs were normalized by dividing by post-challenge day (PCD) 0 frequencies. Statistical analysis was performed using the two-way ANOVA with repeated measures and Bonferroni’s correction for multiple comparisons. *P < 0.05 **P < 0.01 ***P < 0.001.