Glycoprofilea
|
M%/D% ratio, Sha31
|
High (> 0.3)
|
High (> 0.3)
|
High (> 0.3)
|
Low (< 0.2)
|
NA
|
N%, Sha31
|
High (> 8%)
|
High (> 8%)
|
High (> 8%)
|
Low (< 8%)
|
NA
|
N-terminus levelb
|
Normal PK: 12B2/Sha31 ratio
|
High (0.8–1.3)
|
Interm. (0.3–0.7)
|
Low (< 0.2)
|
Low (< 0.2)
|
NA
|
High PK: P4/SAF84 ratio
|
High (1.4-4)
|
Interm. (0.2–1.3)
|
ND
|
ND
|
NA
|
High PK: A’/A ratio, SAF34 + Bar224
|
High (> 0.3)
|
High (> 0.3)
|
ND
|
Low (0.8–1.3)
| minute (≪ 0.8) |
kDa (N-band)c
|
Triplex-WB, Sha31
|
19.9–21.6
|
19.3–20.6
|
18.5–19.5e
|
18.5–19.5
|
NA
|
ISS-WB, SAF84
|
17.5
|
17.5
|
16–17
|
16–17
|
8
|
Triplet profile, SAF84/Sha31 ratio at 22– 24 kDa
|
Single
|
Single
|
Double
|
Single
|
Absent
|
Core stability: SAF84 and L42
|
Low
|
Low
|
Low
|
High
|
NA
|
PrP C-terminus ~ 154–234d
|
Present
|
Present
|
Present
|
Present
|
Absent
|