Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Langeveld, Jan P. M.; Pirisinu, Laura; Jacobs, Jorg G.; Mazza, Maria; Lantier, Isabelle; Simon, Stéphanie; Andréoletti, Olivier; Acin, Cristina; Esposito, Elena; Fast, Christine; Groschup, Martin; Goldmann, Wilfred; Spiropoulos, John; Sklaviadis, Theodoros; Lantier, Frederic; Ekateriniadou, Loukia; Papasavva-Stylianou, Penelope; van Keulen, Lucien J. M.; Acutis, Pier-Luigi; Agrimi, Umberto; Bossers, Alex; Nonno, Romolo

doi:10.1186/s13567-019-0718-z

Veterinary Research

Table 1 Goat sample codes and details and final outcome of the TSE typing study

From: Four types of scrapie in goats differentiated from each other and bovine spongiform encephalopathy by biochemical methods

Study code	Country	Identity #	Breed [region]^a	Age (year)^a	Genotype^b	Molecular TSE-type
STUDY CASES
I2	IT	114921/1/1	Camosciata [Piedmond]	10	240PP	CS-2
I3		121429/1/1	Meticcia [Sicily]	5	240PP	CS-2
I4		128710/1/1	Saanen [Lombardy]	3	211QR, 240PS	CS-2
I5		17646/1/1	Meticcia [Sicily]	5	240PP	CS-2
I7		85788/1/1	Meticcia [Sicily]	> 1.5	240PP	CS-2
I9		85792/1/1	Meticcia [Sicily]	6	143HR, 240PS	CS-2
I11		117463/1/1	Meticcia [Emilia-Romagna]	9	240PS	CS-2
I12		144508/1/1	Alpina [Apulia]	5	240PS	CS-2
I15		87016/1/1	Meticcia [Campania]	6	154RH, 240PS	AS
N1	NL	577277	Dwarf goat [Limburg]	2–3	143HR, 240PS	CS-1
N2		586632-32	Dwarf goat [Limburg]	?	240PP	CS-1
N3		586632-33	Dwarf goat [Limburg]	?	143HR, 240PS	CS-1
F2*	FR	CP40	Saanen [INRA]	4	240PS	CS-1
F3		CDP1028	Saanen [Poitou]	5	240PP	CS-1
F6		CP2119	Saanen [Charentes]	3–4	240PS	CS-1
F10		CP/2143	Alpine [Limousin]	3–4	240PS	CS-1
F11*		CP2154	Saanen [Poitou]	4	142IM, 240PP	CS-1
F14		CP9041	Alpine [Poitou]	6	142IM, 240PS	CS-1^c
F16		CP9135	Alpine [Indre]	6	240PS	CS-2
ic-gtBSE1*		CH1075	Saanen [INRA]	?	211RQ, 240PS	BSE
S2	SP	C-163P	Alpine [Asturia]	6	240PS	CS-1
S3	SP	C-645P	Crossbreed [Aragon]	4	240PP	CS-1
G2	GR	1663	Capra prisca [Macedonia]	?	240PP	CS-1^c
G3		1676	Capra prisca [Macedonia]	4	143HR, 240PP	CS-1
G11		GR005	Capra prisca [Larissa]	6	211RQ, 222QK	CS-1
G12		GR177	Capra prisca [Larissa]	4	222QK	<^c
G13		GR018	Capra prisca [Larissa]	5	wt	CS-1
G14		GR055	Capra prisca [Ioannina]	4	wt	CS-1
G15		GR195	Capra prisca [Kozani]	2.5	222QK	NEG
G16		GR091	Capra prisca [Thessaloniki]	2	wt	CS-1
G17		GR247	Capra prisca [Evros]	4.6	222QK	NEG
C1	CYP	Zyp13	Damascus [Nicosia]	4	240PP	CS-1
C2		Zyp21	Damascus [Nicosia]	5	240PP	CS-1
C3		Zyp27	Damascus [Nicosia]	3	wt, 240PP	CS-1
UK-A2	UK	G08-1475	Anglo-NubianxSaanen [?]	4	127GS, 240PP	CS-1
UK-B2		G08-1469	Anglo-NubianxSaanen [?]	8	127GS, 240PP	CS-CH1641
UK-C2		G08-1460	Anglo-NubianxSaanen [?]	9	127GS, 240PP	CS-1
UK-D2		G08-1446	Anglo-NubianxSaanen [?]	7	211RQ, 240PP	<^c

Study codes will be used in the text reflecting the country of origin, I for Italy, N Netherlands, F France, S Spain, G Greece, C Cyprus, UK United Kingdom. From single holdings were only the cases from Netherlands, UK and Greek cases G13 and G16. Symbols: *, obtained after experimental infections (see “Materials and methods”). The specific tests performed on the samples, the PrP^res content of the sample (when analysed in Triplex-WB) are presented in Additional file 2.
^aINRA, Institut National de la Recherche Agronomique. ? = region of origin or age not disclosed or not known.
^bGenotype as defined by specific polymorphic codon positions in the goat PRNP gene. Wild type (wt) is defined as follows: 127GG, 142II, 143RR, 154RR, 211RR, 222QQ, 240SS. Unless wt genotype, only the codon positions that differ from homogenous wild type are shown.
^cSamples F14 and G2: probably CS-1. F14 too weak for Triplex-WB and ISS-PK methods, G2 too little amount for distribution. G12 and UK-D2: late samples, only analysed by Triplex-WB, too weak signal for analysis.

Back to article page

ISSN: 1297-9716

Contact us

Submission enquiries: veterinary.research@inrae.fr
General enquiries: info@biomedcentral.com