Figure 2
Triplex-WB of goat study samples from different geographical regions together with TSE controls. Three antibodies used are indicated left. Images are all taken from the same blotting membrane. Lanes P and M, respectively recombinant shPrP and molecular mass standards. Position of molecular mass standards are visible only in the 647 nm (12B2) image and are indicated with kDa figures. Sample identities as in Tables 1 and Additional files 1 and 2 are indicated above the lanes. Only the CH1641 controls and UK-B2 sample exhibit a unique glycoprofile difference between SAF84 and Sha31. In lane C-gtCH1641, the positions of the three bands in triplets PrPres#1 (black, D1, M1, N1) and PrPres#2 (blue, D2, M2, N2) are indicated in the SAF84 panel at the right, including their approximate molecular masses; M1 and D2 have nearly similar molecular masses. Therefore, the signal of SAF84 in the D2 + M1 area is clearly higher than in the D1 area because this co-migration reflects the sum of D2 and M1 with that antibody, while with antibodies having more N-terminus located epitope specificity than SAF84 such as Sha31 the D1-area is higher than the D2 + M1 region.” Tissue equivalents applied vary between 0.5 and 2 mg. Samples were analysed in triplicate WB experiments.