SjFrzb2 mRNA profiles detected by qRT-PCR. A A comparison of SjFrzb2 transcript levels at 7, 14, 18, 21, 25, 28, 35, and 42 dpi. B SjFrzb2 mRNA expression profiles in female worms and male worms at 18, 21, 25, 28, 35, and 42 dpi. C SjFrzb2 mRNA expression profile at 25 days (d) in SF (25-day-old female worms from a single-sex infection) and 25 days in MF (25-day-old female worms from a bisexual infection). S. japonicum β-tubulin was used as an internal control. All experiments were performed in triplicate, *p < 0.05, **p < 0.01 vs. the control group.