Figure 4

TEM of E. multilocularis metacestodes incubated in vitro with 3-BrPA. A, B The metacestodes of DMSO control show an intact morphology. LL: laminated layer, GL: germinal layer, Teg: tegument, Uc: undifferentiated cells, Gly: glycogen storage cells. The arrows indicate microtriches originating from the tegument and protruding into the germinated layer. C, D The metacestodes incubated with 40 μM 3-BrPA for 120 h show severe structural damages. The microtriches are shortened and reduced dramatically and even disappeared in most parts of the tegument. The germinal layer tissues show degeneration, largely filled with vacuoles (black arrows) and electron-dense mitochondria (mi).