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Figure 2 | Veterinary Research

Figure 2

From: In vitro and in vivo effects of 3-bromopyruvate against Echinococcus metacestodes

Figure 2

In vitro effects of the selected glycolytic inhibitors against E. multilocularis metacestodes. A Damage upon vesicles resulted from the selected drugs was assessed by measuring the E. multilocularis AP (EmAP) activities in the medium supernatant after 36 and 120 h of in vitro treatment. All drugs were added to a final concentration of 40 μM. Vesicles incubated in culture medium containing DMSO served as controls. *p < 0.05 vs. ornidazole, clorsulon, sodium oxamate, 2,6-dihydroxynaphthalene, medium and DMSO groups. B In vitro concentration-dependent EmAP activities after 120 h incubation with different concentrations (5 to 100 μM) of 3-BrPA. C Visualization of metacestodes after 36 h treatment of 40 μM 3-BrPA. (Panel a) Vesicles incubated in culture medium containing DMSO exhibit a normal morphological structure; (panel b) Metacestodes incubated in culture medium containing 40 μM 3-BrPA exhibit the loss of turgor, collapse of the vesicles, and detachment of the germinal layer from the laminated layer. LL: laminated layer, GL: germinal layer.

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