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Table 2 Overview of the efficacy data

From: Efficacy of three innovative bacterin vaccines against experimental infection with Mycoplasma hyopneumoniae

ParameterStudy dayNCGPCGLipo_DDA:TDBPLGA_TLRSWE_TLRP value
RDS−6 until 560.01 ± 0.010.68 ± 0.32b0.42 ± 0.33ab0.33 ± 0.35ab0.26 ± 0.15a0.011
−6 until 270.00 ± 0.000.00 ± 0.00a0.00 ± 0.00a0.00 ± 0.00a0.00 ± 0.01a0.415
28 until 560.01 ± 0.031.47 ± 0.69b0.90 ± 0.73ab0.73 ± 0.77ab0.56 ± 0.33a0.011
ADG in g/pig/d0 until 56691 ± 69655 ± 109a610 ± 90a628 ± 97a688 ± 49a0.065
0 until 28620 ± 47592 ± 104a560 ± 104a586 ± 106a581 ± 70a0.526
28 until 56762 ± 98739 ± 141ab660 ± 96a669 ± 127a795 ± 69b0.004
Number of animals with macroscopic lung lesions (% positive animals)560/5 (0.00)11/11 (100.00)7/12 (58.33)7/12 (58.33)7/12 (58.33)
Macroscopic lung lesion score560.00 ± 0.007.57 ± 5.18b2.28 ± 3.55a1.43 ± 2.31a0.88 ± 1.01a0.002
Microscopic lung lesion score561.13 ± 0.183.73 ± 0.94b2.11 ± 0.89a2.22 ± 0.87a1.78 ± 0.57a< 0.001
Percentage of lung area occupied by air5639.11 ± 6.2623.38 ± 6.43b37.57 ± 5.99a34.82 ± 4.28ab42.15 ± 7.98a< 0.001
Number of animals positive for M. hyopneumoniae in BAL fluid (% positive animals)420/511/11 (100.00)9/11 (81.82)9/12 (75.00)6/12 (50.00)
560/58/11 (72.73)5/12 (41.67)6/12 (50.00)4/12 (33.33)
Log copies M. hyopneumoniae DNA/mL BAL fluid420.00 ± 0.003.82 ± 0.71b2.50 ± 1.18ab2.20 ± 1.38a1.25 ± 1.28a< 0.001
560.00 ± 0.001.74 ± 0.94a1.24 ± 1.34a1.76 ± 1.68a0.63 ± 0.74a0.082
  1. Pigs were prime-boost vaccinated on D0 and D14 with three different experimental M. hyopneumoniae bacterins (Lipo_DDA:TDB, PLGA_TLR, SWE_TLR), challenge infected on D28–29 and euthanized on D56. For the parameters ADG, macroscopic lung lesion score, microscopic lung lesion score, % air and number of M. hyopneumoniae organisms in BAL fluid, a Kruskal–Wallis ANOVA was performed to determine statistical differences between the groups on each time point. For the parameter RDS, a repeated measurements ANOVA with Scheffé’s post hoc test was performed. The NCG was not included in the statistical analyses. Groups that have no superscript in common are significantly different from each other (P ≤ 0.05). All results are expressed as mean ± SD, unless otherwise stated.
  2. NCG: non-challenge control group (non-vaccinated, non-challenge infected), PCG: PBS-injected control group (non-vaccinated, challenge infected), RDS: respiratory disease score, ADG: average daily gain, BAL: bronchoalveolar lavage.