Cytokine concentrations (pg/mL) in bronchoalveolar lavage fluid. Pigs were prime-boost vaccinated on D0 and D14 with three different experimental M. hyopneumoniae bacterins (Lipo_DDA:TDB, PLGA_TLR, SWE_TLR), challenge infected on D28–29 and euthanized on D56. IL-1β (A), IL-6 (B), IFN-γ (C) and TNF-α (D) concentrations in BAL fluid were measured using commercial sandwich ELISAs. For each time point, significance was calculated using a Kruskal–Wallis ANOVA. The NCG was not included in the statistical analyses. Groups that have no superscript in common are significantly different from each other (P ≤ 0.05). NCG, non-challenge control group (PBS-injected, non-challenge infected), PCG, PBS-injected control group (PBS-injected, challenge infected), BAL, bronchoalveolar lavage.