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Table 1 Diagnostic primers for porcine RVC, RVA and RVB and cloning primers for porcine RVC full length structural genes, VP7, VP6, VP4 and VP2

From: Rotavirus C: prevalence in suckling piglets and development of virus-like particles to assess the influence of maternal immunity on the disease development

Target or function

Sequence

Region (nt)

Reference/source

Detection

 RVA

Forward: 5′-ACCATCTACACATGACCCTC-3′

Reverse: 5′-GGTCACATAACGCCCC-3′

963–982

[62]

 RVB

Forward: 5′-GGTTTAAATAGCCCAACCGACGC-3′

Reverse: 5′GTRTTYAAATTSGTRTTTGGCGCT-3′

1–94

[19]

 RVC

Forward: 5′-ATGTAGCATGATTCACGAATGGG-3′

Reverse: 5′-ACATTTCATCCTCCTGGGGAT-3′

Probe: 5′-VIC-GCG TAG GGG CAA ATG CGC ATG A-TAMRA-3′

 

[19]

RVC amplification

 VP7

G1 and G3 forward: 5′-ATG GTT TGT ACR ACA TTG TRC 3′

G1 and G3 reverse: 5′-GCC ACA TGA TCT TGT TTA CGC GTA T-3′

G6 forward: 5′-ATG GTT TGT ACA ACT TTG TAC AC AGT TT-3′

G6 reverse: 5′-GCC ACA TGA TCT TGT TTA CGC GTA TC-3′

1–1054

This study

 VP6

G1 and G3 G6 forward: 5′-ATG GAT GTA CTT TTT TCY ATY GCG-3′

G1, G3 and G6 reverse: 5′-TTC GCC CTT AGC CAC ATA GTT CAC ATT TC-3′

1–1294

This study

 VP4

G1, G3 and G6 forward: 5′-GGA TCA ATG GCG TCC TC-3′

G1, G3 and G6 reverse: 5′-AGC CAC ACA ATC AGT CGA TCT CCT CAC-3′

1–2232

This study

 VP2

G1 and G3 forward: 5′-ATG ATA AGC AGG AAY AGA C-3′

G1 and G3 reverse: 5′-CAG AAT TTG AGG TCR TCA CAA GAT-3′

G6 forward: 5-ATG ATA AGC AGG AAT AGA-3′

G6 reverse: 5′-GAA TTT GAG GTC ATC ACA AGA TG-3′

1–2737

This study