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Table 1 Diagnostic primers for porcine RVC, RVA and RVB and cloning primers for porcine RVC full length structural genes, VP7, VP6, VP4 and VP2

From: Rotavirus C: prevalence in suckling piglets and development of virus-like particles to assess the influence of maternal immunity on the disease development

Target or function Sequence Region (nt) Reference/source
Detection
 RVA Forward: 5′-ACCATCTACACATGACCCTC-3′
Reverse: 5′-GGTCACATAACGCCCC-3′
963–982 [62]
 RVB Forward: 5′-GGTTTAAATAGCCCAACCGACGC-3′
Reverse: 5′GTRTTYAAATTSGTRTTTGGCGCT-3′
1–94 [19]
 RVC Forward: 5′-ATGTAGCATGATTCACGAATGGG-3′
Reverse: 5′-ACATTTCATCCTCCTGGGGAT-3′
Probe: 5′-VIC-GCG TAG GGG CAA ATG CGC ATG A-TAMRA-3′
  [19]
RVC amplification
 VP7 G1 and G3 forward: 5′-ATG GTT TGT ACR ACA TTG TRC 3′
G1 and G3 reverse: 5′-GCC ACA TGA TCT TGT TTA CGC GTA T-3′
G6 forward: 5′-ATG GTT TGT ACA ACT TTG TAC AC AGT TT-3′
G6 reverse: 5′-GCC ACA TGA TCT TGT TTA CGC GTA TC-3′
1–1054 This study
 VP6 G1 and G3 G6 forward: 5′-ATG GAT GTA CTT TTT TCY ATY GCG-3′
G1, G3 and G6 reverse: 5′-TTC GCC CTT AGC CAC ATA GTT CAC ATT TC-3′
1–1294 This study
 VP4 G1, G3 and G6 forward: 5′-GGA TCA ATG GCG TCC TC-3′
G1, G3 and G6 reverse: 5′-AGC CAC ACA ATC AGT CGA TCT CCT CAC-3′
1–2232 This study
 VP2 G1 and G3 forward: 5′-ATG ATA AGC AGG AAY AGA C-3′
G1 and G3 reverse: 5′-CAG AAT TTG AGG TCR TCA CAA GAT-3′
G6 forward: 5-ATG ATA AGC AGG AAT AGA-3′
G6 reverse: 5′-GAA TTT GAG GTC ATC ACA AGA TG-3′
1–2737 This study