Virus-like particles generation. A Sf9 cells were co-infected with six different genotype (G1, G3 and G6) combinations recombinant baculovirus expressing RVC core and outer capsid protein genes shown in Table 2. At day 7 the VLPs were harvested and semi-purified through sucrose were gradient. Analysis of semi-purified VLPs was done using a 12% precast protein gel. Lanes were loaded as follows: (1) VP7 G3, VP4 G3, VP6 G6, VP2 G6. (2) VP7 G6, VP4 G6, VP6 G6, VP2 G6. (3) VP7 G1, VP4 G1, VP6 G1, VP2 G1. (4) VP7 G3, VP4 G3, VP6 G1, VP2 G1. (5) VP7 G1, VP4 G1, VP6 G6, VP2 G6 and (6) VP7 G6, VP4 G6, VP6 G1, VP2 G1. B EM image of porcine RVC VLP G6 genotype produced by infecting Sf 9 cells with recombinant baculoviruses containing ORFs coding for porcine RVC VP2, VP4, VP6 and VP7. Scale bar 100 nm.