Figure 2

Porcine RVC structural protein expression. A Amplification of porcine RVC G1, G3 and G6 structural genes; VP7, VP6, VP4 and VP2 using specific primers. Amplicons were analyzed using 1% agarose gel. Expected sizes were 1054 bp for VP7, 1293 bp for VP6, 2432 bp for VP4 and 2724 bp for VP2 structural genes. B Sf9 cells were transfected with RVC recombinant baculovirus DNA containing individual porcine RVC structural genes (VP2, VP4, VP6, and VP7) from three strains G1, G3 and G6 at day 6 showing CPE. C Western blot of VP7, VP6, VP4 and VP2 protein expression using hyper immune antiserum for G1 as the primary antibody and anti-swine IgG HRP conjugated secondary antibody. The blot was developed using 3,3′,5,5′-tetramethylbenzidine (TMB) substrate.