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Table 1 List of primers used for qRT-PCR

From: Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway

S. No. Gene name Primers (from 5′ to 3′) Product length
1 TLR2-A Forward 5′-TCGCTCCAACACCTTCGCATTC
Reverse 5′-GATTGTCACCGTCGATCCTCAGC
181
2 TLR2-B Forward 5′-TCCTCATCCTGGTGGTCGTTGG
Reverse 5′-GGCTTCCGCTTGGCTTGGAG
88
3 TLR4 Forward 5′-TTCGGTTGGTGGACCTGAATCTTG
Reverse 5′-ACAGCTTCTCAGCAGGCAATTCC
114
4 NLRP3 Forward 5′-GCTCCTTGCGTGCTCTAAGACC
Reverse 5′-TTGTGCTTCCAGATGCCGTCAG
150
5 IL-10 Forward 5′-CAGCACCAGTCATCAGCAGAGC
Reverse 5′-GCAGGTGAAGAAGCGGTGACAG
94
6 IL-18 Forward 5′-AGATGATGAGCTGGAATGCGATGC
Reverse 5′-ATCTGGACGAACCACAAGCAACTG
97
7 PTGE Forward 5′-GCCTTCTACAGCACGATCCTGATC
Reverse 5′-GCCTTCTTCCTGAGCCTCACTTG
80
8 ALOX5 Forward 5′-GCGGTTCACAATAGCCATCAACAC
Reverse 5′-GCTGTAGGTCAGGTCCTTCATTGC
139
9 ALOX15B Forward 5′-GTGAAGGAGCGGACAGTGAAGTG
Reverse 5′-AACCAGGCATCCTCCAGGAAGAG
95
10 LOXHD1 Forward 5′-CACAGACAAGACCTTCCGCTTCC
Reverse 5′-GCAGTCCGTTCCTTCAGTTCCAG
123
11 IFN-γ Forward 5′-TTCCTGATGGCGTGAAGAAGGTG
Reverse 5′-TCGGAGGATCCACCAGCTTCTG
129
12 GSDMA Forward 5′-AGCCTCACAGAAGCCATCTCCTAC
Reverse 5′-GCTGCTGCTGCTCGCTGAAG
196
13 GSDME Forward 5′-GCTGCGTGCCTGCTCTGATC
Reverse 5′-GCTCAGTGCCAAGGTGCCATC
88
14 TRAF2 Forward 5′-CGTGGTGATGAAAGGACCCA
Reverse 5′-AATGATGTGCTCCCGGTTGT
100
15 TNF-R1 Forward 5′-CCTGTCTGTCTTCCCTGTCC
Reverse 5′-GGTGCATGGGGTCTTTTCTA
120
16 TRADD Forward 5′-CTAGAGCCCAAAGGAAGTCGAT
Reverse 5′-TGGCTGCTTCTCTGTGACAT
100
17 FADD Forward 5′-GGGGTAAAGAGGCTGAACTCTTA
Reverse 5′-TGAGTCCTATTGCACTGCTGTC
163
18 Nrf2 Forward 5′-GATGTCACCCTGCCCTTAGA-3′
Reverse 5′-TCGTTCCATTTGTTCCTTCTG-3′
124
19 GPX-1 Forward 5′-AAGTGCTGCTGGTGGTCAACG
Reverse 5′-GTTGGTGGCGTTCTCCTGGTG
155
20 GPX-3 Forward 5′-GTGGCAGAGGAGTTCGGCAAC
Reverse 5′-TCTTGACAGTGGCGATGTTGGC
151
21 PRDX6 Forward 5′-GCATCCGCTTCCACGACTTCC
Reverse 5′-GGCGTTGATGTCCTTGCTCCAG
200
22 MAP1LC3 Forward 5′-GCTGCCAGTGCTGGACAAGAC
Reverse 5′-TCCTCATCCTTCTCCTGCTCGTAG
189
23 Beclin-1 Forward 5′-ACCGCAAGATTGTGGCTGAAGAC
Reverse 5′-TGAGCATAACGCATCTGGTTCTCC
163
24 mTOR Forward 5′-AACCACTGCTCGCCACAATGC
Reverse 5′-CATAGGATCGCCACACGGATTAGC
120
25 HO-1 Forward 5′-TCATTGGCAAGAAGCATCCAGAGC-3′
Reverse 5′-GAACTTGGTGGCGTTGGAGACTC-3′
176
26 NF-κB Forward 5′-CACATGGTGGTGACCGCCAATAG-3′
Reverse 5′-GTGCCATCGTATGTAGTGCTGTCC-3′
194
27 TNF-α Forward 5′-TGATCGTGACACGTCTCTGC-3′
Reverse 5′-CAACCAGCTATGCACCCCAG-3′
88
28 IL-6 Forward 5′-TTCACCGTGTGCGAGAACAGC-3′
Reverse 5′-CAGCCGTCCTCCTCCGTCAC-3′
80
29 IL-1β Forward 5′-AGCAGCCTCAGCGAAGAGACC-3′
Reverse 5′-GTCCACTGTGGTGTGCTCAGAATC-3′
90
30 Bax Forward 5′-ACTCTGCTGCTGCTCTCCTCTC-3′
Reverse 5′-ATCCACGCAGTGCCAGATGTAATC-3′
174
31 Caspase-3 Forward 5′-TACCGGACTGTCATCTCGTTCAGG-3′
Reverse 5′-ACTGCTTCGCTTGCTGTGATCTTC-3′
166
32 Caspase-8 Forward 5′-GGAAGCAGTGCCAGAACTCAGAAG-3′
Reverse 5′-TTGTTGTGGTCCATGCACCGATAG-3′
174
33 Caspase-9 Forward 5′-CCGAAGGAGCAAGCACGACAG-3′
Reverse 5′-CATCTAGCATGTCAGCCAGGTCAC-3′
121
34 P53 Forward 5′-GGAGATGGAACCATTGCTGGAACC-3′
Reverse 5′-GCTCCTGCCAGTTGCTGTGATC-3′
113
35 Bcl2 Forward 5′-GAGTTCGGCGGCGTGATGTG-3′
Reverse 5′-TTCAGGTACTCGGTCATCCAGGTG-3′
92
36 Cytochrome C Forward 5′-CCTAATCGCCGTGGCCTTCTTAAC-3′
Reverse 5′-GGAGGAGGTAGATGGTCGGATTGG-3′
163
37 NQO1 Forward 5′-TCGCCGAGCAGAAGAAGATTGAAG-3′
Reverse 5′-GGTGGTGAGTGACAGCATGGC-3′
191
38 GSTA2 Forward 5′-GGAGTCAATCCGGTGGCTGTTAG-3′
Reverse 5′-GGCTCTGCTCTGCACCATCTTC-3′
163
39 ATG5 Forward 5′-GGACGCATACCAACCTGCTT
Reverse 5′-TGCCATTTCAGTGGCGTACC
200
40 Dynein Forward 5′-CGTTGCCAGCGTTACACCTATCC
Reverse 5′-GCCAGGACTGCCACCAACAC
163
41 F-actin Forward 5′-ACCTGGATTGGAGAGGATGTCAGC
Reverse 5′-CGGCCTTCTTCAGCTCGTTCTTG
160
42 α-Tubulin Forward 5′-GCGGCACGGCAAGTACATGG
Reverse 5′-CTTGGTCTTGATGGTGGCGATGG
94
43 TGFβ1 Forward 5′-GCCGACACGCAGTACACCAAG
Reverse 5′-GCAGGCACGGACCACCATATTG
168
44 β-actin Forward 5′-CAACACAGTGCTGTCTGGTGGTAC-3′
Reverse 5′-CTCCTGCTTGCTGATCCACATCTG-3′
199