Evaluation of the immunogenicity and efficacy of BCG and MTBVAC vaccines using a natural transmission model of tuberculosis

Table 1 Number of positive reactors in each experimental group using different diagnostic tests and antigens

Test	Antigen	Group	T0^a	T1	T3^b	T5	T7	T9	T11	T12
IGRA	PPD-B cut-off 0.05	Control	0/17	0/17	0/17	2/17	9/15	12/14	10/12	10/12
		BCG	0/17	2/17	6/17	0/17	3/15	7/14	10/14	8/14
		MTBVAC	0/17	14/17	7/17	1/16	5/15	11/13	11/13	12/13
	PPD-B cut-off 0.1	Control	0/17	0/17	0/17	2/17	8/15	12/14	10/12	10/12
		BCG	0/17	2/17	5/17	0/17	1/15	3/14	7/14	6/14
		MTBVAC	0/17	12/17	4/17	0/16	3/15	8/13	6/13	9/13
	E/C^c	Control	0/17	0/17	NA	2/17	5/15	11/14	6/12	7/12
		BCG	0/17	0/17	NA	0/17	0/15	0/14	2/14	1/14
		MTBVAC	0/17	3/17	NA	1/16	0/15	4/13	1/13	5/13
	Rv3615^c	Control	0/17	0/17	NA	1/17	3/15	8/14	3/12	3/12
		BCG	0/17	0/17	NA	0/17	0/15	0/14	0/14	0/14
		MTBVAC	0/17	1/17	NA	0/16	0/15	1/13	1/13	3/13
SIT test	PPD-B	Control	NA	NA	1/17	2/17	NA	11/14	NA	11/12
		BCG	NA	NA	15/17	4/17	NA	2/14	NA	11/14
		MTBVAC	NA	NA	15/17	1/16	NA	8/13	NA	12/13
	E/C	Control	NA	NA	NA	2/17	NA	9/14	NA	6/12
		BCG	NA	NA	NA	1/17	NA	2/14	NA	2/14
		MTBVAC	NA	NA	NA	0/16	NA	5/13	NA	5/13
SCIT test	PPD-B and PPD-A	Control	NA	NA	0/17	2/17	NA	7/14	NA	10/12
		BCG	NA	NA	10/17	1/17	NA	1/14	NA	8/14
		MTBVAC	NA	NA	11/17	0/16	NA	6/13	NA	9/13
P22_ELISA (E% 100)	P22 and PPD-A	Control	0/17	0/17	0/17	0/17	3/15	2/14	8/12	6/12
		BCG	0/17	0/17	0/17	3/17	5/15	2/14	11/14	13/14
		MTBVAC	0/17	1/17	0/17	3/16	8/15	1/13	12/13	10/13
P22_ELISA (E% 150)	P22 and PPD-A	Control	0/17	0/17	0/17	0/17	2/15	2/14	4/12	6/12
		BCG	0/17	0/17	0/17	2/17	2/15	1/14	4/14	5/14
		MTBVAC	0/17	1/17	0/17	0/16	3/15	0/13	4/13	8/13

E%, ELISA percentage for interpretation and cut-off point; E/C, cocktail of ESAT-6/CFP-10; IGRA, interferon-gamma release assay; NA, not available; PPD-B, bovine purified protein derivative; PPD-A, avian purified protein derivative; SIT, single intradermal tuberculin test; SCIT, single comparative intradermal tuberculin test.
^aVaccination (time in months).
^bExposure to infected animals.
^cCut-off 0.1.

ISSN: 1297-9716