Recent advances in delivery of veterinary DNA vaccines against avian pathogens

Jazayeri, Seyed Davoud; Poh, Chit Laa

doi:10.1186/s13567-019-0698-z

Veterinary Research

Table 1 Efficacy of DNA vaccines against poultry pathogens delivered by different carriers

From: Recent advances in delivery of veterinary DNA vaccines against avian pathogens

Pathogens	Host/day of vaccination	Vehicle	Target antigen/adjuvant	Route of vaccination	Immune responses	Protection	Ref.
AIV	Chicken/3 and 6 week	Naked plasmid	H6 + Kozak sequence	IM	Anti-HA Ab	Not tested	[32]
AIV	Chicken/10, 24 and 38 day	Naked plasmid	H5 and HSP-70	IM	Anti-HA Ab	Not tested	[13]
AIV	Chicken/7, 21 and 35 day	Naked plasmid	H5 and Esat-6	IM	Anti-HA Ab	Not tested	[10]
AIV	Chicken/1 and 14 day	Naked plasmid	N1 and IL15	IM	Anti-N1 Ab/CD4 and CD8	Not tested	[9]
ILTV	Chicken/3 and 5 week	Naked plasmid	gB and IL18	IM	Anti-ILTV Ab and CD4 and CD8	80%	[84]
IBV	Chicken/7, 21 and 35 day	Naked plasmid	S1, N, and M	IM	IBV-neutralizing Ab and CD4/CD8	90%	[85]
NDV	Chicken/21, 36 and 46 day	Naked plasmid	HN, F, and IL4	IM	Anti-NDV Ab and cellular immune responses	40%	[34]
AIV	Chicken/3, 7 and 11 week	Naked plasmid + liposome	H5	IM	Anti-HA Ab	Not tested	[27]
AIV	Chicken/3 and 6 week	pHEMA	H6	IM	Ab response	Reduced virus shedding	[86]
C. psittaci	Turkey/1 and 3 week	Branched PEI	OmpA	IM	IgG and increased CD4/CD8 rate response	Reduced C. psittaci shedding and shortened the period of clinical signs	[77]
Anatid herpesvirus 1	Duck/4 week	Chitosan and liposome	gC	IM	Rapid and extensive plasmid distribution in duck tissues	Not tested	[87]
NDV	Chicken/1 week	Lipofectin	F and HN	IM	Anti-F Ab	80%	[88]
Coccidiosis	Chicken/1 and 2 week	Naked plasmid	Gam56 from Eimeria maxima	IM	Ab and lymphocyte proliferation	No chicken died from E. tenella challenge in any group	[89]
IBDV	Chicken/2 and 4 week	Naked plasmid	VP2 + HSP70	IM	Anti-VP2 Ab and lymphocyte proliferation	100%	[14]
CIA	Chicken/3, 5 and 7 week	Naked plasmid	VP1 and VP2 + HMGB1ΔC	IM	Anti-CIA Ab and CD8	Not tested	[12]
NDV	Chicken/30 and 44 day	Chitosan nanoparticles	F	IM/IN	IgA/IgG and lymphocyte proliferation	IM: 80% IN: 100%	[90]
REV	Chicken/3 and 6 week	Naked plasmid	gp90 + WPRE	IM	Lymphocyte proliferation, Ab response and IL-4 and IFN-γ	87%	[91]
P. multocida	Chicken/4, 6 and 8 week	Naked plasmid	OmpH and OmpA	IM	Induction of CD8 T cells, high serum Ab titres, and IFN-γ	70%	[92]
Coccidiosis	Chicken/2 and 3 week	Naked plasmid	TA4 and chicken IL-2	IM	Humoral response	~80%	[93]
Anatid herpesvirus 1	Duck/4 week	Naked plasmid + chitosan DNA and liposome	gC	IM	Extensive plasmid distribution in duck tissues	Not tested	[87]
DEV	Duck/10 and 12 week	Naked plasmid	gD and B	IM	Stimulated a high frequency of CD4, CD8 T cells and neutralizing antibody	70%	[94]
TCoV	Turkey/1, 7 and 21 day	Naked plasmid +PEI and sodium hyaluronate	4F/4R	IM	Anti-TCoV S Ab and VN titre	Decrease in clinical signs from 5/5 to 1/5 or 2/5	[95]
Coccidiosis	Chicken/4, 14 and 21 day	Naked plasmid	EtMIC2 + IL-18	IM	Anti-EtMic2 Ab, CD8/CD4 T cells	No chicken died from E. tenella challenge in any group	[96]
AIV	Quail/3, 6 and 9 week	Naked plasmid	H5	IM	Anti-HA Ab	100%	[21]
NDV	Chicken/30 and 44 day	Nano-chitosan	F	IM/IN	IgG and IgA and lymphocyte proliferation	80% (IM); 100% (IN)	[26]
IBDV	Egg embryonation on day 18 and at 1 week	Naked plasmid/killed vaccine	VP2, VP3, VP4 + killed virus booster	IO/IM	Anti-IBDV Ab and lymphocyte proliferation	100%	[43]
DPV	Duck/3 week	Naked plasmid +gold particles	gC	IM + GG	Increase the numbers of CD4, CD8 T cells and neutralizing antibody	Not tested	[97]
AVA	Chicken/6 and 13 day	S. typhimurium SL7207	pVAX-rC	Oral	Ab response	66.7%	[66]
AIV	Chicken/2 and 4 week	S. typhimurium SL7207	H1	Oral	Anti-HA intestinal mucosal IgA response and lymphoproliferation	100% (combined with killed vaccine)	[65]
Coccidiosis	Chicken/3 and 17 day	S. typhimurium SV4089	5401	Oral	Ab and cellular immune responses	55–57.5%	[62]
IBDV	Chicken/7 and 12 day	S. typhimurium SV4089	VP2/4/3	Oral	Ab response	73.3%	[61]
AIV	Chicken/1 day	S. typhimurium SV4089	H1	Oral	Anti-HA Ab and CD4/CD8 T cells	Not tested	[19]
CAV	Chicken/2, 4 and 6 week	L. acidophilus	VP1	Oral	Anti-CAV Ab/cell mediated responses	Not tested	[71]
IBDV	Chicken/7 and 14 day	Transgenic E. coli DH5α	VP2	Oral	Anti-IBDV Ab	95.4%	[54]
AIV	Chicken/1 day	NanoAg-poly(ethylene glycol)	H5	Oral	Anti-HA Ab and CD4/CD8 T cell response	Not tested	[20]
AIV	Chicken/10 and 24 day	Lactobacillus (LDL17-pH)	H1	Oral	Anti-HA mucosal IgA and serum IgG l, IFNγ, TLR-2, and AvBD-9 in the PPs and CTs	60%	[72]
DEV	Duck/3 times per day for 7 day	S. typhimurium	UL24 + labile enterotoxin B subunit	Oral	Serum IgA and IgG, DEV-specific mucosal IgA and anti-NA Ab	60-80%	[67]
DTMUV	Duck/8 and 24 day	S. typhimurium SL7207	prM and E	Oral	Specific antibody against the E protein and VN	100%	[68]
IBDV	Chicken/2 and 16 day	S. enterica serovar Typhimurium	S1 and N	Oral/IN	Humoral and mucosal immune responses	70%	[25]
C. jejuni	Chicken/1, 15 and 29 day	Nano-chitosan	Protein FlaA	IN	IgG and intestinal mucosa IgA responses	Not tested	[79]
M. gallisepticum	Chicken/2 week	M. gallisepticum ts-11	ts-11 C3 and IFN-γ	ED	Cell-mediated immunity	Not tested	[98]
IBDV	Chicken/3, 5 and 7 week	PLGA	VP2	ED	Stimulation of CD4 and CD8 T cells, high level of IgG	80%	[78]
AIV	Chicken/1 and 28 day	Naked plasmid	H7	IV, IP, and SC	Anti-HA Ab	50%	[99]
AIV	Chicken/3 week	Naked plasmid	H5	GG	Anti-HA Ab	100%	[16]
AIV	Duck/5 and 8 week	Naked plasmid	H5	External thigh muscle, Medi Jector Vision	NV Ab	Not tested	[100]
NDV and IBDV	Egg embryonation at 14–18 day	Plasmid/neutral lipid/DMSO	HA, VP2, VP4 and VP3	IO	Expression of viral protein	Not tested	[36]
NDV	Egg embryonation at 18 day	Naked plasmid	F and HN	IO	Anti-HA Ab	28%	[42]
IBV	Egg embryonation at 18 day	Naked plasmid/IFNα	gS	IO	Humoral immune responses	89%	[101]
ILTV	Egg embryonation at 18 day	Naked plasmid	CpG	IO	IgM, KUL01, and CD8 and CD4 cells	~40%	[41]

Ref: references, S1: spike protein, N: nucleocapsid protein, M: membrane protein, Ab: antibody, ILTV: infectious laryngotracheitis virus, g: glycoprotein, pHEMA: poly(2-hydroxyethyl methacrylate), REV: reticuloendotheliosis virus, TCoV: turkey coronavirus, DPV: duck plague virus, AVA: avian viral arthritis, PPs: Peyer’s patches, CTs: caecal tonsils, VN: virus neutralization, GG: gene gun, IV: intravenous, IP: intraperitoneal, SC: subcutaneous, d: day, wk: week.

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