Figure 7

BEFV inhibits Bcl-2 binding to Beclin 1. A Cells were infected with BEFV at a MOI of 1 followed by reciprocal co-immunoprecipitation assays with the Bcl-2 or Beclin 1 antibodies. In reciprocal co-immunoprecipitation assays, anti-Beclin 1 rabbit IgG or anti-Bcl-2 mouse IgG were used as negative controls, respectively. B Overexpression of Bcl 2 suppresses BEFV production. BHK-21 and BHK-21/Bcl-2 cells were infected with BEFV at a MOI of 1. The cell lysate was collected at 18 hpi and subjected to immunoblotting for detecting the levels of M protein and LC3 II. The levels of the indicated proteins in the mock controls (un-infection) were considered onefold. The protein levels were normalised to those for β-actin. The activation and inactivation folds indicated below each lane were normalized against values for the mock control. Signals were quantified using Image J software. The predicted size of each protein was labeled at the right-hand side in kDa.