Figure 2

Stable transfection of A549 cells with NMBR shRNA. A Bright-field microscopy of cells transfected with shRNA-Luciferase (1, mock cells; 3, sh-NMBR cells); GFP-expressing cells were successfully transfected with shRNA-Luciferase (2, mock cells; 4, sh-NMBR cells). The expression of NMBR was assessed by RT-PCR (B), qRT-PCR (C) and WB (D). β-Actin or GAPDH was used for internal standardization. All images were captured at ×200 magnification, scale bars = 200 μm, **P < 0.05.