Figure 6

Fluorescence and Raman images of a rectal epithelial cell containing bLF derived from milk. Analysis from a cell in the absence of EHEC. A Fluorescence microscopy image of bLF-Alexa Fluor 488 in the cell. B The heat map generated using the 1555 cm⁻¹ peak, which is highly intense in bLF. C The heat map generated using the 1003 cm⁻¹ peak of phenylalanine, showing the protein distribution inside the cell. D Raman scattering spectra showing the peaks used for generation of the heat maps (B, C). The upper, red spectrum taken inside the nucleus, in the area marked with red rectangle on (B) and (C), contains the peak at 1555 cm⁻¹. The bottom, blue spectrum, obtained outside the nucleus, in the area marked with blue rectangle on (B) and (C), has no 1555 cm⁻¹ peak.