Figure 3
N. caninum burdens in placental and foetal tissues. Comparative of parasite burdens quantified by qPCR in nested-PCR positive samples from CA and CO (A) and FB and FL (B) from G-NcSpain7 and G-NcSpain1H heifers culled at 10 and 20 dpi. C Comparative of parasite quantification by qPCR between CA and CO and between FB and FL from G-NcSpain7 heifers culled at 20 dpi. D Comparative of parasite quantification by qPCR in samples from CA, CO, FB and FL from NVF and VF foetuses from G-NcSpain7 culled 20 dpi. Each dot represents individual values of parasite burden, and medians are represented as horizontal lines. The N. caninum detection limit by real-time PCR was 0.1 parasites, and negative samples (0 parasites) were represented on the log scale as < 0.1 (i.e., 10−2). ****, ***, and * indicate P < 0.0001, P < 0.001 and P < 0.05 significant differences. σ indicates P < 0.1 tendency towards significant differences.