Table 2 Trichinella spiralis proteins involved in molting that are upregulated in 10 h IIL
Protein name | Protein IDs | Gene name | Theor. MW (kDa) | IIL/ML | P value | Unique peptides | Coverage (%) | GO annotations |
|---|---|---|---|---|---|---|---|---|
Cuticle structural elements | ||||||||
 Cuticle collagen 14 | E5RZT0 | Tsp_11095 | 32.91 | 23.00 | 0.0107 | 5 | 15.60 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Putative nematode cuticle collagen N-domain protein | E5SQP6 | Tsp_09393 | 59.89 | 8.12 | 0.0496 | 4 | 6.30 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Cuticle collagen rol-6 | E5SQP9 | Tsp_09396 | 39.15 | – | – | 3 | 13.60 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Cuticle collagen lon-3 | A0A0V1BMR6 | lon-3 | 43.45 | – | – | 3 | 12.20 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Cuticle collagen dpy-7 | E5SR67 | Tsp_09560 | 29.37 | – | – | 6 | 26.60 | CC: collagen trimer |
 Cuticle collagen 6 Protein roller-8 | E5SAG9 | rol-8 | 35.18 | – | – | 3 | 9.50 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Cuticle collagen sqt-1 | A0A0V1BL19 | sqt-1 | 34.73 | – | – | 10 | 40.00 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Putative cuticle collagen 2 | E5S488 | Tsp_03639 | 26.52 | – | – | 3 | 21.10 | CC: collagen trimer; integral component of membrane; MF: structural constituent of cuticle |
 Putative PAN domain protein | E5S7X4 | Tsp_07811 | 67.36 | 15.61 | 0.0001 | 14 | 36.20 | BP: cuticle pattern formation; embryonic ectodermal digestive tract morphogenesis; CC: cytoplasmic side of apical plasma membrane; external side of apical plasma membrane; extracellular space; supramolecular fiber; MF: structural constituent of cuticle |
Regulators of cuticle synthesis, maintenance, remodeling and degradation | ||||||||
 Zinc carboxypeptidase superfamily | E5SP83 | Tsp_09758 | 55.37 | – | – | 5 | 14.00 | MF: metallocarboxypeptidase activity; zinc ion binding |
 Putative zinc metalloproteinase nas-13 | E5S4Y1 | Tsp_05948 | 19.96 | – | – | 2 | 11.80 | – |
 Metalloendopeptidase | A0A0V1C237 | TFE3 | 99.70 | – | – | 16 | 23.50 | BP: protein complex oligomerization; protein maturation by iron-sulfur cluster transfer; MF: metalloendopeptidase activity; zinc ion binding |
 Cathepsin B | S5M797 | – | 37.40 | 2.29 | 0.0208 | 2 | 7.90 | MF: cysteine-type peptidase activity |
 Cathepsin F | E5SFB3 | Tsp_02382 | 41.90 | 3.26 | 0.0145 | 8 | 25.40 | MF: cysteine-type peptidase activity |
Attachment components | ||||||||
 Transmembrane cell adhesion receptor mua-3 | A0A0V1BV71 | mua-3 | 402.52 | 3.14 | 0.0326 | 1 | 3.10 | CC: collagen-containing extracellular matrix; integral component of membrane; MF: calcium ion binding; extracellular matrix structural constituent |
 Transmembrane matrix receptor MUP-4 | A0A0V1BTR4 | mup-4 | 280.00 | 2.03 | 0.0159 | 49 | 27.90 | CC: collagen-containing extracellular matrix; integral component of membrane; MF: calcium ion binding; extracellular matrix structural constituent |
 E3 ubiquitin-protein ligase listerin | A0A0V1B0D3 | ltn1 | 201.88 | 2.34 | 0.0007 | 25 | 19.40 | BP: ribosome-associated ubiquitin-dependent protein catabolic process; CC: RQC complex; MF: ligase activity; ubiquitin protein ligase activity |
Hormonal regulation of molting | ||||||||
 Cytochrome P450 4V2 | E5SY27 | Tsp_10837 | 57.31 | 8.59 | 0.0001 | 22 | 47.40 | CC: integral component of membrane; MF: heme binding; iron ion binding; monooxygenase activity; |
 Putative nuclear hormone receptor HR3 | A0A0V1AYJ5 | Hr46 | 53.37 | – | – | 1 | 2.70 | CC: nucleus; MF: nuclear receptor activity; sequence-specific DNA binding; steroid hormone receptor activity |
Intracellular trafficking components and regulators | ||||||||
 Low-density lipoprotein receptor-related protein | A0A0V1B0U4 | lrp-1 | 74.61 | – | – | 13 | 31.00 | CC: integral component of membrane; calcium ion binding |
 Serine/threonine-protein kinase RIO1 | A0A0V1BAH6 | Riok1 | 59.34 | – | – | 3 | 7.00 | MF: ATP binding; protein serine/threonine kinase activity |