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Table 1 Primers used in the quantitative real-time PCR assays

From: Label-free quantitative proteomic analysis of molting-related proteins of Trichinella spiralis intestinal infective larvae

Gene description NCBI accession Primer sequence Product size (bp)
Cuticle collagen 14 (E5RZT0) XM_003381945.1 F 5′-TCTGGTGATGCATCGGATCG-3′
R 5′-TCCTTCAGCACACGCTTCAT-3′
113
Cytochrome P450 4V2 (E5SY27) XM_003370180.1 F 5′-CGCAAACTGCTCACACCATC-3′
R 5′-CGCAGATGATGTCCAAAGCG-3′
172
Endoplasmic oxidoreductin-1 (E5SKW7) XM_003373203.1 F 5′-AGGTAATGCGAACAAGGCGA-3′
R 5′-ATGGGTCTGCAACTTTCCCC-3′
124
Putative DOMON domain-containing protein (E5SFI2) XM_003378229.1 F 5′-GCTGATTCCGCTTCTCCAGT-3′
R 5′-CGGGTCTGGTTTTTCGCTTG-3′
157
Glutamine synthetase (E5SIC2) XM_003374954.1 F 5′-CCCGTTTCGACTGGGAAAGA-3′
R 5′-CGAGATCGAGCAGCGTGTAT-3′
167
RNA-binding protein 47 (E5S1I0) XM_003378983.1 F 5′-CGCAATACGCGTCCAAGAAG-3′
R 5′-AGAGACCGATGATGGTGGGA-3′
174
Cathepsin F (E5SFB3) XM_003378197.1 F 5′-ATGGCCCCACAATTTTTGCC-3′
R 5′-TCCATTCGCTCGCTGATGTT-3′
133
Isocitrate dehydrogenase, NADP-dependent (E5S5M3) XM_003380431.1 F 5′-TGTACTCATGTGCCCGGATG-3′
R 5′-GGGGTTCGTTTCGATCCAGT-3′
176
GAPDH (Reference) AF452239 F 5′-GATGCTCCTATGTTGGTTATGGG-3′
R 5′-GTCTTTTGGGTTGCCGTTGTAG-3′
196