Table 1 Primers used in the quantitative real-time PCR assays
Gene description | NCBI accession | Primer sequence | Product size (bp) |
|---|---|---|---|
Cuticle collagen 14 (E5RZT0) | XM_003381945.1 | F 5′-TCTGGTGATGCATCGGATCG-3′ R 5′-TCCTTCAGCACACGCTTCAT-3′ | 113 |
Cytochrome P450 4V2 (E5SY27) | XM_003370180.1 | F 5′-CGCAAACTGCTCACACCATC-3′ R 5′-CGCAGATGATGTCCAAAGCG-3′ | 172 |
Endoplasmic oxidoreductin-1 (E5SKW7) | XM_003373203.1 | F 5′-AGGTAATGCGAACAAGGCGA-3′ R 5′-ATGGGTCTGCAACTTTCCCC-3′ | 124 |
Putative DOMON domain-containing protein (E5SFI2) | XM_003378229.1 | F 5′-GCTGATTCCGCTTCTCCAGT-3′ R 5′-CGGGTCTGGTTTTTCGCTTG-3′ | 157 |
Glutamine synthetase (E5SIC2) | XM_003374954.1 | F 5′-CCCGTTTCGACTGGGAAAGA-3′ R 5′-CGAGATCGAGCAGCGTGTAT-3′ | 167 |
RNA-binding protein 47 (E5S1I0) | XM_003378983.1 | F 5′-CGCAATACGCGTCCAAGAAG-3′ R 5′-AGAGACCGATGATGGTGGGA-3′ | 174 |
Cathepsin F (E5SFB3) | XM_003378197.1 | F 5′-ATGGCCCCACAATTTTTGCC-3′ R 5′-TCCATTCGCTCGCTGATGTT-3′ | 133 |
Isocitrate dehydrogenase, NADP-dependent (E5S5M3) | XM_003380431.1 | F 5′-TGTACTCATGTGCCCGGATG-3′ R 5′-GGGGTTCGTTTCGATCCAGT-3′ | 176 |
GAPDH (Reference) | AF452239 | F 5′-GATGCTCCTATGTTGGTTATGGG-3′ R 5′-GTCTTTTGGGTTGCCGTTGTAG-3′ | 196 |