Results of LC–MS/MS of ML and 10 h IIL proteins. A The cartoon showing the intersection of differentially expressed T. spiralis proteins between the ML and 10 h IIL. Twenty-nine proteins were found solely in ML (■) and 116 only in 10 h IIL (■). Of the identified proteins, 2562 co-existed in both ML and IIL without a significant difference in expression level (■), 78 were obviously upregulated in the ML (■), and 100 were upregulated in IIL (■). B The plot exhibiting the distribution of quantified proteins based on their statistical significance (P value) and fold change. The proteins over the dotted line are considered statistically significant (P < 0.05), and those beyond the two vertical dotted lines show 2.0-fold changes compared with the ML stage. Differentially expressed proteins between the two larval stages are represented as red dots.