VtaA2 and VtA2OL are produced under in vitro conditions. Detection by immunoblot of VtaA2 and its truncated version VtaA2OL from E. coli BL21 (pLGR-vtaA2) and E. coli BL21 (pLGR-vtaA2OL) respectively. Both proteins were extracted from bacterial pellets grown in LB-Cm30 or incubated with CDMEM. Molecular size standards in kDa are shown on the left. Protein extraction from E. coli BL21 transformed with pACYC184 empty plasmid was used as negative control of expression.