The potential of acetylsalicylic acid and vitamin E in modulating inflammatory cascades in chickens under lipopolysaccharide-induced inflammation

Table 2 Genes and primers used in this study

Gene	Primer	Sequence (5′–3′)	Melting temperature (°C)	Product size (nt)	GenBank access no.
ACTB	Forward	TGTTACCAACACCCACACCC	60.11	110	NM 205518.1
ACTB	Reverse	TCCTGAGTCAAGCGCCAAAA	60.18	110	NM 205518.1
COX-1	Forward	GCGCATCAGTAGACCTAGCC	60.32	121	JX160009.1
COX-1	Reverse	TGGTATTGTGACAGTGCGGG	60.32	121	JX160009.1
COX-2	Forward	TGTCCTTTCACTGCTTTCCAT	57.77	84	NM_001167718
COX-2	Reverse	TTCCATTGCTGTGTTTGAGGT	58.11	84	NM_001167718
LOX-12	Forward	CTGATTACGCCGTGCTGGAT	60.53	73	XM_015274997.1
LOX-12	Reverse	ATTGGGGCACACAGGAATGT	59.89	73	XM_015274997.1
CYP450p	Forward	ACCACTTCTGGAAGGAGGGA	59.81	108	D49803.1
CYP450p	Reverse	CGCTCTCGTAGACACCCAAC	60.46	108	D49803.1

ACTB: β-actin, COX-1: cyclooxygenase-1, COX-2: cyclooxygenase-2, LOX-12: lipoxygenase-12, and CYP450p: cytochrome P450.

ISSN: 1297-9716