Figure 6
Survival efficiency of the wild-type, ΔnorV mutant, and complemented CΔnorV strains in RAW264.7 macrophages. A CFU of intracellular bacteria at 1 h post-infection (time point 0). B Intracellular survival rate of A. hydrophila in macrophages. Phagocytosis was allowed to proceed for 1 h at a MOI of 1.0. The CFU of intracellular bacteria was estimated every 20 min. The relative survival was calculated by dividing the mean CFU at a specific time point by the CFU at time point 0. The survival rate at time point 0 was arbitrarily set to 100%. Data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01.