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Figure 5 | Veterinary Research

Figure 5

From: Diverse effects of nitric oxide reductase NorV on Aeromonas hydrophila virulence-associated traits under aerobic and anaerobic conditions

Figure 5

The norV is diversely involved in secretion of Hcp protein. A Hcp proteins in the culture supernatants of bacteria grown under aerobic and anaerobic conditions after exposure with various concentrations of SNP were determined by Western blot. Polyclonal anti-Hcp antibodies were used to measure the secretion of Hcp and anti-GroEL antibodies served as internal reference. Lines 1, 4, 7, 10 represent the wild type strain NJ-35; Lines 2, 5, 8, 11 represent the ΔnorV mutant; and Lines 3, 6, 9, 12 represent the complemented CΔnorV strain. The relative changes of Hcp secretion of bacteria grown under aerobic (B) and anaerobic (C) conditions are given between the blots. Values are the means ± SD from three biological replicates. *P < 0.05, **P < 0.01, ***P < 0.001.

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