Figure 1
The expression of norV in response to NO donor (sodium nitroprusside, SNP) under aerobic and anaerobic conditions. The A. hydrophila strains were cultured to OD600 of 0.8 with various concentrations of NO donor under aerobic and anaerobic conditions. A The mRNA level of norV in the wild type strain determined by qRT-PCR. The expression value of norV without SNP treatment in the aerobic condition was normalized to 1.0. Values are means ± SD from three biological replicates. Different uppercase letters (a–g) indicate significant differences (P < 0.05) among different treatments. B The expression levels of NorV protein detected by Western blot. Lines 1, 4, 7, 10 represent the wild type strain NJ-35; Lines 2, 5, 8, 11 represent the ΔnorV mutant; and Lines 3, 6, 9, 12 represent the complemented CΔnorV strain.