Figure 5
Transcriptional response of Marc-145 cells to xanthohumol treatment. RNA profiling by RNA sequencing of Marc-145 cells treated with 10 µM Xn or DMSO for 4, 8, and 12 h. A Waterfall plot of the up- and downregulated genes at each time point (DEGs selected based on padj < 0.05). B Unsupervised hierarchical cluster analysis of DEGs. DEG expression levels are represented as FPKM-normalized log2-transformed counts. Blue indicates low relative expression, and red indicates high relative expression. C Venn diagram of the unique and shared genes of each group. The cluster number for each component is listed. D Significantly enriched KEGG terms of the 462 shared genes