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Figure 3 | Veterinary Research

Figure 3

From: Xanthohumol inhibits PRRSV proliferation and alleviates oxidative stress induced by PRRSV via the Nrf2–HMOX1 axis

Figure 3

Xanthohumol inhibits PRRSV early in infection in Marc-145 cells. A Time-of-addition schematic. Marc-145 cells were infected with PRRSV (0.01 MOI) for 1 h (0 to 1 h), and cells were treated with 10 µM Xn at different times of infection, designated pre-treatment (pre), co-treatment (co), or post-treatment (post). B The effect of Xn was determined by CPE and IFA. C The effect of different concentrations of Xn was detected by Western blotting. D Xn (10 µM) or DMSO was incubated with PRRSV (0.001, 0.01, and 0.1 MOI) for 2 h at 37 °C and then inoculated into Marc-145 cells. After incubation for 48 h at 37 °C, the viral titres were detected by TCID50 assays. The results are representative of three independent experiments performed in triplicate

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