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Table 1 Primer list for 1st round PCR and 2nd round PCR for Tn-seq

From: Tn-sequencing of Mycoplasma hyopneumoniae and Mycoplasma hyorhinis mutant libraries reveals non-essential genes of porcine mycoplasmas differing in pathogenicity

No

Primer

Sequence

1

NGS_1st_Seq6

GACTTGAGCGTCGATTTTTGTG

2

NGS_1st_PCR_rev_1

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-TGATT

3

NGS_1st_PCR_rev_2

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-TTGAT

4

NGS_1st_PCR_rev_3

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-TTTAG

5

NGS_1st_PCR_rev_4

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-CAGG

6

NGS_1st_PCR_rev_5

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-TTCCC

7

NGS_1st_PCR_rev_6

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-NNNNNNNNNN-TCAGC

8

Seq7_NGS_tail_F

TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-GTTGCGGTACCCTTTTACAC

9

NGS_tail_R

GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG

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Veterinary Research

ISSN: 1297-9716