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Table 4 The details of the primary antibodies used in this study

From: Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome

NameHostDilution ratioCat. no.Manufacturer
IL-1 βRabbit1:1000ASC0912Thermo Fisher, USA
IL-6Goat1:500PP690
IL-8Goat1:600AF535R&D Systems, USA
TNF-αGoat1:600AF690
p-IκBαMouse1:5009246CST, USA
TLR4Rabbit1:50019811-1-APProteintech, Wuhan
MyD88Rabbit1:50023230-1-AP
p65Rabbit1:50010745-1-AP
IκBαRabbit1:50010268-1-AP
TBPMouse1:50066166-1-lg
GAPDHMouse1:500060004-1-lg
NLRP3Rabbit1:500/Kindly gift by Prof. Weng, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences
Caspase-1Rabbit1:1000/
ASCMouse1:500/
ASCRabbit1:200bs-6741RBioss, Beijing