Table 4 The details of the primary antibodies used in this study
Name | Host | Dilution ratio | Cat. no. | Manufacturer |
|---|---|---|---|---|
IL-1 β | Rabbit | 1:1000 | ASC0912 | Thermo Fisher, USA |
IL-6 | Goat | 1:500 | PP690 | |
IL-8 | Goat | 1:600 | AF535 | R&D Systems, USA |
TNF-α | Goat | 1:600 | AF690 | |
p-IκBα | Mouse | 1:500 | 9246 | CST, USA |
TLR4 | Rabbit | 1:500 | 19811-1-AP | Proteintech, Wuhan |
MyD88 | Rabbit | 1:500 | 23230-1-AP | |
p65 | Rabbit | 1:500 | 10745-1-AP | |
IκBα | Rabbit | 1:500 | 10268-1-AP | |
TBP | Mouse | 1:500 | 66166-1-lg | |
GAPDH | Mouse | 1:5000 | 60004-1-lg | |
NLRP3 | Rabbit | 1:500 | / | Kindly gift by Prof. Weng, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences |
Caspase-1 | Rabbit | 1:1000 | / | |
ASC | Mouse | 1:500 | / | |
ASC | Rabbit | 1:200 | bs-6741R | Bioss, Beijing |