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Table 2 Experimental design for the induction of the inflammatory response in PAMs

From: Matrine inhibits IL-1β secretion in primary porcine alveolar macrophages through the MyD88/NF-κB pathway and NLRP3 inflammasome

 Cell control group (Cell)Transfection reagents group (RNA-0)5′UTR RNA transfect group (RNA-n)5′UTR RNA + LPS group (RNA-n + LPS)LPS control group (LPS)
Transfection reagents+++
5′UTR RNA++
1 μg/mL LPS++
  1. n represents the RNA dose used for transfection, n = 1, 2 or 4 μg.