Effect of matrine on TLR4, DHX36 and MyD88 expression. PAMs from all treatment groups were collected, and total RNA or protein was extracted and assessed by qRT-PCR or Western blot, respectively. A The expression of the TLR4, DHX36 and MyD88 genes. The results showed no changes in TLR4 gene expression levels among the different groups (p > 0.05), but DHX36 and MyD88 gene expression levels were significantly increased in the 5′UTR RNA and LPS co-stimulation group (p < 0.05). Compared with the co-stimulation group, the matrine-treated group showed reduced expression of DHX36 and MyD88 genes (p < 0.05). B The expression of TLR4, DHX36 and MyD88 protein levels. Expression was normalized to that of GAPDH. #Indicates that the expression was significantly different compared to that of the group with 5′UTR RNA and LPS co-stimulation (p < 0.05).