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Table 1 Bacterial strains and plasmids used in this study

From: Interleukin-1 signaling induced by Streptococcus suis serotype 2 is strain-dependent and contributes to bacterial clearance and inflammation during systemic disease in a mouse model of infection

Strain/plasmid General characteristics Source/references
Streptococcus suis
 P1/7 Classical virulent serotype 2 ST1 strain isolated from a pig with meningitis in the UK [69]
 P1/7Δsly Isogenic mutant strain derived from P1/7; in frame deletion of sly gene This work
 P1/7Δlgt Isogenic mutant strain derived from P1/7; in frame deletion of lgt gene [31]
 SC84 Highly virulent serotype 2 ST7 strain isolated from a human case of streptococcal toxic shock-like syndrome during the 2005 human outbreak in China [70]
 SC84Δsly Isogenic mutant strain derived from SC84; in frame deletion of sly gene This work
 SC84Δlgt Isogenic mutant strain derived from SC84; in frame deletion of lgt gene [31]
Escherichia coli
 TOP 10 F mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
 BL21 FompT hsdSB (r B , m B ) gal dcm rne131 (DE3) Invitrogen
Plasmids
 pIVEX2.4d Apr, pUC ori, T7 promoter, His tag-coding sequence Roche Bioscience
 pSET4Δsly pSET4 s carrying the construct of sly gene for allelic replacement This work
 pIVEXsly pET101 carrying sly gene for protein production This work