Table 1 Bacterial strains and plasmids used in this study
Strain/plasmid | General characteristics | Source/references |
|---|---|---|
Streptococcus suis | ||
 P1/7 | Classical virulent serotype 2 ST1 strain isolated from a pig with meningitis in the UK | [69] |
 P1/7Δsly | Isogenic mutant strain derived from P1/7; in frame deletion of sly gene | This work |
 P1/7Δlgt | Isogenic mutant strain derived from P1/7; in frame deletion of lgt gene | [31] |
 SC84 | Highly virulent serotype 2 ST7 strain isolated from a human case of streptococcal toxic shock-like syndrome during the 2005 human outbreak in China | [70] |
 SC84Δsly | Isogenic mutant strain derived from SC84; in frame deletion of sly gene | This work |
 SC84Δlgt | Isogenic mutant strain derived from SC84; in frame deletion of lgt gene | [31] |
Escherichia coli | ||
 TOP 10 | F− mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
 BL21 | F−ompT hsdSB (r −B , m −B ) gal dcm rne131 (DE3) | Invitrogen |
Plasmids | ||
 pIVEX2.4d | Apr, pUC ori, T7 promoter, His tag-coding sequence | Roche Bioscience |
 pSET4Δsly | pSET4 s carrying the construct of sly gene for allelic replacement | This work |
 pIVEXsly | pET101 carrying sly gene for protein production | This work |