|
Streptococcus suis
|
|
P1/7
|
Classical virulent serotype 2 ST1 strain isolated from a pig with meningitis in the UK
|
[69]
|
|
P1/7Δsly
|
Isogenic mutant strain derived from P1/7; in frame deletion of sly gene
|
This work
|
|
P1/7Δlgt
|
Isogenic mutant strain derived from P1/7; in frame deletion of lgt gene
|
[31]
|
|
SC84
|
Highly virulent serotype 2 ST7 strain isolated from a human case of streptococcal toxic shock-like syndrome during the 2005 human outbreak in China
|
[70]
|
|
SC84Δsly
|
Isogenic mutant strain derived from SC84; in frame deletion of sly gene
|
This work
|
|
SC84Δlgt
|
Isogenic mutant strain derived from SC84; in frame deletion of lgt gene
|
[31]
|
|
Escherichia coli
|
|
TOP 10
|
F− mrcA Δ(mrr-hsdRMS-mcrBC) φ80 lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu) 7697 galU galK rpsL (StrR) endA1 nupG
|
Invitrogen
|
|
BL21
|
F−ompT hsdSB (r
−B
, m
−B
) gal dcm rne131 (DE3)
|
Invitrogen
|
|
Plasmids
|
|
pIVEX2.4d
|
Apr, pUC ori, T7 promoter, His tag-coding sequence
|
Roche Bioscience
|
|
pSET4Δsly
|
pSET4 s carrying the construct of sly gene for allelic replacement
|
This work
|
|
pIVEXsly
|
pET101 carrying sly gene for protein production
|
This work
|
