Figure 4
Role of Toll-like receptors (TLRs) and associated signaling in S. suis-induced IL-1β production by bone marrow-derived dendritic cells (bmDCs). A Percentage of IL-1β production induced by S. suis strain P1/7 (white bars) or SC84 (gray bars) 16 h following infection of bmDCs deficient for MyD88, TRIF, TLR2 or TLR4, with regards to wild-type counterparts (normalized to 100%); B IL-1β production 24 h following activation of wild-type (WT) or TLR2−/− bmDCs with 30 μg/mL of LTA extracts from strains P1/7 or SC84 or their lgt-deficient mutants (Δlgt); C IL-1β production by bmDCs following phagosomal delivery of 1 µg of S. suis RNA or DNA; D Percentage of IL-1β production from bmDCs following pre-treatment with inhibitors of either NF-kB, p38, MEK or JNK and infection with S. suis, with regards to vehicle-treated bmDCs (DMSO; normalized to 100%). Data are expressed as mean ± SEM (n = 3). #p < 0.05) indicates a significant difference with wild-type bmDCs (normalized to 100%); $(p < 0.05) indicates a significant difference with negative control (elution buffer); *(p < 0.05) indicates a significant difference with vehicle-treated bmDCs (normalized to 100%).