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Table 3 Viral attachment to E-11 cells at 25 °C

From: Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase

Viral strain

Inoculum3

Adsorbed virus4

25 °C

30 °C

RNA1 copies1

Log ± SD2

RNA1 copies

Log ± SD

Ad rate5

RNA1 copies

Log ± SD

Ad rate

wt160

4.10 × 1011

11.61 ± 0.14

4.08 × 1011

11.61 ± 0.31

99.51

4.07 × 1011

11.61 ± 0.31

99.26

r160

7.24 × 1010

10.86 ± 0.15

7.19 × 1010

10.86 ± 0.15

99.31

7.17 × 1010

10.85 ± 0.15

99.03

r1_445

3.22 × 1011

11.51 ± 0.25

3.19 × 1011

11.50 ± 0.24

99.07

3.18 × 1011

11.50 ± 0.24

98.75

RGNNV

5.62 × 1010

10.75 ± 0.18

5.60 × 1010

10.75 ± 0.08

99.64

5.58 × 1010

10.75 ± 0.08

99.28

SJNNV

1.71 × 1011

12.23 ± 0.15

1.70 × 1011

11.23 ± 0. 27

99.41

1.69 × 1011

11.23 ± 0.15

98.83

  1. Results expressed as RNA1 copies/mL (1) and log10RNA1 copies/mL ± standard deviation (2) from 3 replicas of inoculated (3) and adsorbed virus (estimated as the difference between the RNA1 values of the original inocula and those of the remaining inocula after 1 h adsorption) (4) The efficiency of the adsorption (5; as percentage) was calculated from the ratio between adsorbed and total viral inoculum.
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Veterinary Research

ISSN: 1297-9716