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Table 3 Viral attachment to E-11 cells at 25 °C

From: Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase

Viral strain Inoculum3 Adsorbed virus4
25 °C 30 °C
RNA1 copies1 Log ± SD2 RNA1 copies Log ± SD Ad rate5 RNA1 copies Log ± SD Ad rate
wt160 4.10 × 1011 11.61 ± 0.14 4.08 × 1011 11.61 ± 0.31 99.51 4.07 × 1011 11.61 ± 0.31 99.26
r160 7.24 × 1010 10.86 ± 0.15 7.19 × 1010 10.86 ± 0.15 99.31 7.17 × 1010 10.85 ± 0.15 99.03
r1_445 3.22 × 1011 11.51 ± 0.25 3.19 × 1011 11.50 ± 0.24 99.07 3.18 × 1011 11.50 ± 0.24 98.75
RGNNV 5.62 × 1010 10.75 ± 0.18 5.60 × 1010 10.75 ± 0.08 99.64 5.58 × 1010 10.75 ± 0.08 99.28
SJNNV 1.71 × 1011 12.23 ± 0.15 1.70 × 1011 11.23 ± 0. 27 99.41 1.69 × 1011 11.23 ± 0.15 98.83
  1. Results expressed as RNA1 copies/mL (1) and log10RNA1 copies/mL ± standard deviation (2) from 3 replicas of inoculated (3) and adsorbed virus (estimated as the difference between the RNA1 values of the original inocula and those of the remaining inocula after 1 h adsorption) (4) The efficiency of the adsorption (5; as percentage) was calculated from the ratio between adsorbed and total viral inoculum.