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Table 2 Cytopathic effect (CPE) observed in E-11 cells inoculated with the mutated virus r1_445, the 160 strain (wt and r) and parental strains from genotypes RGNNV and SJNNV at different temperatures

From: Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase

Days pi

Temperature (°C)

15

20

25

30

RGNNV

SJNNV

wt160

r160

r1_445

RGNNV

SJNNV

wt160

r160

r1_445

RGNNV

SJNNV

wt160

r160

r1_445

RGNNV

SJNNV

wt160

r160

r1_445

1

S

S

S

S

S

2

S

S

S

S

S

+

3

S, D

S

S

S

S

+

+

+

+

+

++

+

+

++

+

4

S, D

S

S

S

S

++

+

+

+

+

+++

+

++

+++

+

5

S, D

S

S

S

S

+

+

+

+++

+++

++

++

++

++++

+

++++

++++

+++

6

S, D

S, D

S, D

S

S

++

++

+

+

+

++++

++++

+++

+++

++

NA

+

NA

NA

+++

7

S, D

S, D

S, D

S

S

++

++

++

++

+

NA

NA

++++

++++

+++

NA

+

NA

NA

++++

8

S, D

S, D

S, D

S, D

S

+++

+++

+++

+++

+

NA

NA

NA

NA

+++

NA

++

NA

NA

NA

9

S, D

S, D

S, D

S, D

S

++++

++++

+++

++++

++

NA

NA

NA

NA

++++

NA

+++

NA

NA

NA

10

S, D

S, D

S, D

S, D

S

NA

NA

++++

NA

+++

NA

NA

NA

NA

NA

NA

+++

NA

NA

NA

  1. S: Cell shrinkage, D: detached cells, − absence of cytopathic effect (CPE), + initial vacuolation, ++ extensive vacuolation, +++ extensive vacuolation and initial disruption of the monolayer, ++++ complete destruction of the monolayer, NA: not applicable.