Amino acidic substitutions in the polymerase N-terminal region of a reassortant betanodavirus strain causing poor adaptation to temperature increase

Table 1 Oligonucleotides used for mutagenesis and positions of the point mutations in strain r1_445

Oligonucleotide sequence (5′ to 3′)^a	nt substitution^b	aa substitution
AGGATTATCGCCAACGCGTCATCGCTGAGAAGAAA	A-199 → G	Iso-41 → Val
CGCGTCATCGCTGAGAAGAAACAAATTCTCCGTGATGC	G-220 → A	Val-48 → Iso
CCCGACCTCGAGGTTTCTGGGCGAATC	C-730 → T	Leu-218 → Phe
GAGGTTTCTGGGCGAATCTGATGCAGATCCTG	T-745 → A	Leu-223 → Met
GTCACCGCGATCTGTAGTTTTCTTTACACCAAGC	A-791 → T	Thr-238 → Phe
CAAGATCAGTGAGTATGGTGCTGAGTTGGAGTATATGCG	A-943 → G	Thr-289 → Ala

ISSN: 1297-9716