Virulence of viral strains for Senegalese sole. The curves represent the fish survival after infection by immersion with r160 and the mutant r1_445 at 15, 20 and 25 °C. Fish were infected by bath at a final concentration of 105 TCID50/mL. Values are expressed as mean ± SD (n = 3). Asterisk indicates significant differences (p < 0.05).