Flow cytometric analysis of M. hyopneumoniae (pMHGFP-P97) fluorescence over time. A M. hyopneumoniae (pMHGFP-P97) culture was analysed for green fluorescence over time by flow cytometry. The histogram FL1-A axis depicts green fluorescence intensity. On histograms plots, the vertical line indicates the boundary between fluorescent and non-fluorescent cells, to right of the line are fluorescent cells. A 24 h. B 48 h. C 72 h. D 96 h. E 168 h. F Untransformed M. hyopneumoniae. G Viable counts of M. hyopneumoniae (pMHGFP-P97) were performed from 24 to 96 h. Error bars show standard error of the mean.