Figure 6

IL-17C induce occludin expression in IPEC-J2 cells. IPEC-J2 cells were cultured and differentiated on transwell inserts and then incubated with C83901 at MOI 100 or PBS and IL-17C (20 ng/mL) for 1 h, washed and further incubated for another 23 h. A Cellular localization of occludin in IPEC-J2 cells 24 h post C83901 and IL-17C stimulation. The cells were stained with anti-occludin (Texas-Red, Red) and the nuclei were counterstained with Hoechst and representative confocal images are shown (n = 3). Scale bar = 10 μm. B Occludin protein was detected by Western blotting in IPEC-J2 cells 24 h post C83901 and IL-17C stimulation.