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Figure 5 | Veterinary Research

Figure 5

From: Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection

Figure 5

Gene expression of tight junctions and proinflammatory cytokines and epithelial permeability changes after bacterial infection. IPEC-J2 cells were cultured and differentiated on transwell inserts and then incubated with HB101 and C83901 at MOI 100 or PBS and IL-17C (20 ng/mL) for 1 h, washed and further incubated for another 23 h. A Occludin, ZO-1, TNF-α and IL-8 mRNA expression was assessed by qPCR in the IPEC-J2 monolayers at 2 and 4 h post-infection/stimulation. The mRNA expression level was normalized to the reference genes and then to the control group. B Apical and basolateral IL-8 secretion by differentiated IPEC-J2 cells 24 h post-infection/stimulation. IPEC-J2 monolayers were pre-incubated with TH1020 (0.5 μM) for 2 h. Then, the monolayers were inoculated with HB101 and C83901 at MOI 100 or PBS and IL-17C (20 ng/mL) for 1 h, washed and further incubated for 23 h. C Transepithelial electrical resistance (TEER) was measured in IPEC-J2 cells after bacterial infection or IL-17C stimulation. Different letters indicate significant differences (p < 0.05). Data are presented as the mean ± SD (n = 5 per group).

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