Skip to main content

Advertisement

Figure 3 | Veterinary Research

Figure 3

From: Toll-like receptor 5-mediated IL-17C expression in intestinal epithelial cells enhances epithelial host defense against F4+ ETEC infection

Figure 3

F4+ETEC infection elicits an IL-17C response under the control of TLR5 in IPEC-J2 cells. IPEC-J2 monolayers were first pre-cultured with 0.5 μM TH1020 (0.5 μM), 0.5 μM oligodeoxyribonucleotide (ODN) 2088 or the equivalent amount of their solvent DMSO and TE or PBS for 2 h. Then, the monolayers were inoculated with the C83901 at MOI 100 for 1 h, washed and further incubated for 23 h. A IL-17C cytokine production was analysed in the cell culture supernatant by ELISA. Data are presented as the mean ± SD (n = 5 per group). Different letters indicate significant differences between groups (a:b, p < 0.05; a:c, p < 0.01). B IL-17C protein was detected by Western blotting. C IPEC-J2 monolayers were first pre-cultured with TH1020 (0.5 μM, green bars), DMSO (red bars) or PBS (blue bars) for 2 h. Then, the monolayers were stimulated with flagellin (FLA, 100 ng/mL) and further incubated for 4 h and 24 h for mRNA and protein expression of IL-17C, respectively. Control samples only treated with PBS were marked with open bars. Different letters indicate significant differences between groups (a:b, p < 0.05; a:c, p < 0.01). Data are presented as the mean ± SD (n = 5 per group).

Back to article page