Figure 1

F4⁺ ETEC induces IL-17C and TLR2, 5, 8 and -10 mRNA expression in small intestinal tissues. Jejunal segments of three piglets were perfused with F4⁺ ETEC (C83901), non-pathogenic E. coli strain HB101 or PBS for 4 h. A The mRNA expression of IL-17A, IL-17C and IL-17RE in the intestinal segments was analyzed by qPCR. The mRNA expression level was normalized to the reference genes and then to the control group. Data are presented as the mean ± standard deviation (SD) (n = 3 per group). * p < 0.05. B The mRNA expression of different IL-17 receptors in jejunal segments in homeostatic conditions. The mRNA expression level was normalized to the reference genes and then to IL-17RC. Data are presented as the mean ± SD (n = 6), different letters indicate significant differences between groups (p < 0.01). C TLR mRNA expression profile in jejunal segments after F4⁺ETEC perfusion. Jejunal segments of three piglets were perfused with F4⁺ ETEC (C83901), non-pathogenic E. coli strain HB101 or PBS for 4 h. The mRNA expression of TLR-1 to 10 in the intestinal segments was analyzed by qPCR. The mRNA expression level was normalized to the reference genes and then to the control group. Data are presented as the mean ± SD (n = 3 per group). *p < 0.05, **p < 0.01.