Bacterial colony-forming unit (CFU) counts obtained from mice mammary glands 16 h post-infection with S. aureus 2117. Mammary glands were treated (intra-mammary administration) with the Rhodomyrtus tomentosa leaves extract (RTLex: 300 µg per dose, 2 doses), with pirlimycin (PIR: 1 µg per dose), or a combination of both. For the extract, treatment was done at 0 and 4 h post-infection, whereas pirlimycin was administered at 4 h. PBS was used for the controls (animals infected but not treated). Each dot represents the CFU of individual glands (n = 8–10) and the median value for each group is indicated by the bar. Statistical differences between CFUs recovered from treated and untreated animals are shown (non-parametric Kruskal–Wallis ANOVA with Dunn’s post-test: ***P < 0.001; ****P < 0.0001; ns: not statistically significant (P > 0.05) vs. untreated control).